]]> Place a small piece of tape at one end of the slide Label the tape with the name of the sample Skim Milk broth culture of Klebsiella aerogenes Stir up the Skim Milk broth culture with the loop Place 2-3 loops of Klebsiella aerogenes on the microscope slide Use the loop to spread the sample out to cover about one inch of the slide Let the slide completely air dry Place the slide on a staining tray Cover the entire film with crystal violet Wait 1 minute Pick up the slide by one end and hold it at an angle over the staining tray Wash off the excess dye with 20% copper sulfate solution Shake off the excess copper sulfate solution Use a book of blotting paper to blot the slide dry