]]> Place the entire wire portion of the inoculating loop into the opening of the microincinerator Wait 10 seconds Remove the inoculation loop from the microincinerator Allow the loop to cool 10-20 seconds Pick up the agar plate containing a mixed colony of E. coli and M. luteus Remove a single colony of M. luteus with the loop Pick up the sterile, labeled Trypticase Soy Agar plate Lift the edge of the lid just enough to insert the loop Rotate sector 1 to 12 o'clock position Streak sector 1 Stick the loop into non-steaking area agar to cool it Rotate sector 1 to 9 o'clock position Drag the loop through sector 1 twice Streak sector 2 Rotate sector 2 to 9 o'clock position Drag the loop through sector 2 twice Streak sector 3 Resterilize the inoculating loop by placing it in the microincinerator for 10 seconds Lay the cap on top of the bench Put the loop down