]]> Place the entire wire portion of the inoculating loop into the opening of the microincinerator Wait 10 seconds Remove the inoculation loop from the microincinerator Allow the loop to cool 10-20 seconds Pickup the Mixed bacteria tube Hold the culture tube in one hand and in your other hand, hold the sterilized inoculating loop as if it were a pencil Remove the cap of the pure culture tube with the little finger of your loop hand Place the lip of the culture tube at the opening of the microincinerator for 2-3 seconds Insert the inoculating loop and remove a loopful of inoculum Replace the cap of the culture tube Pick up the sterile, labeled Columbia CNA agar plate Lift the edge of the lid just enough to insert the loop Rotate sector 1 to 12 o'clock position Streak sector 1 Stick the loop into non-steaking area agar to cool it Rotate sector 1 to 9 o'clock position Drag the loop through sector 1 twice Streak sector 2 Rotate sector 2 to 9 o'clock position Drag the loop through sector 2 twice Streak sector 3 Resterilize the inoculating loop by placing it in the microincinerator for 10 seconds Lay the cap on top of the bench Put the loop down Pickup the the sterile, labeled MacConkey agar plate Pick up the sterile, labeled Trypticase Soy Agar plate