]]> Place the entire wire portion of the inoculating loop into the opening of the microincinerator Wait 10 seconds Remove the inoculation loop from the microincinerator Allow the loop to cool 10-20 seconds Pickup the E. coli culture tube Hold the culture tube in one hand and in your other hand, hold the sterilized inoculating loop as if it were a pencil Remove the cap of the pure culture tube with the little finger of your loop hand Place the lip of the culture tube at the opening of the microincinerator for 2-3 seconds Insert the inoculating loop and remove a loopful of inoculum Replace the cap of the culture tube Pick up the sterile Trypticase Soy slant tube Remove the cap of the sterile tube with the little finger of your loop hand Place the lip of the sterile tube at the opening of the microincinerator for 2-3 seconds Place the loopful of inoculum into the tube Place the lip of the innoculated tube at the opening of the microincinerator for 2-3 seconds Replace the cap of the inoculated tube Resterilize the inoculating loop by placing it in the microincinerator for 10 seconds Lay the cap on top of the bench Put the loop down Pickup the B. subtilis culture tube Pickup the M. luteus culture tube Pickup the M. phlei culture tube Pick up the sterile Trypticase Soy broth tube Pick up the sterile Trypticase Soy stab tube Pick up the sterile Trypticase Soy Agar plate