]]> Turn on the microincinerator Wait 10 minutes Place the entire wire portion of the inoculating loop into the opening of the microincinerator Wait 10 seconds Remove the inoculation loop from the microincinerator Allow the loop to cool 10-20 seconds Hold the culture tube in one hand and in your other hand, hold the sterilized inoculating loop as if it were a pencil Remove the cap of the pure culture tube with the little finger of your loop hand Place the lip of the culture tube at the opening of the microincinerator for 2-3 seconds Insert the inoculating loop and remove a loopful of inoculum Replace the cap of the culture tube Lay the cap on top of the bench Put the loop down